منابع مشابه
Preparation of DNA and protein micro arrays on glass slides coated with an agarose film.
A thin layered agarose film on microscope slides provides a versatile support for the preparation of arrayed molecular libraries. An activation step leading to the formation of aldehyde groups in the agarose creates reactive sites that allow covalent immobilization of molecules containing amino groups. Arrays of oligonucleotides and PCR products were prepared by tip printing. After hybridizatio...
متن کاملEnumerating bacterial cells on bioadhesive coated slides.
Quantifying bacterial abundance and biomass is fundamental to many microbiological studies. Directly counting via epifluorescence microscopy has become the method of choice, especially for environmental samples, and conventional techniques require filtration of cells onto black polycarbonate membrane filters. We investigated the utility of instead capturing stained bacterial suspensions on bioa...
متن کاملdevelopment of polymer-coated glass slides as optical oligonucleotide microarrays
background: the microarray technology is in needed of cost-effective, low background noise and stable substrates for successful hybridization and analysis. methods: in this research, we developed a three-dimentional stable and mechanically reliable microarray substrates by coating of two polymeric layers on standard microscope glass slides. for fabrication of these substrates, a thin film of ox...
متن کاملDevelopment of Polymer-Coated Glass Slides as Optical Oligonucleotide Microarrays
BACKGROUND The microarray technology is in needed of cost-effective, low background noise and stable substrates for successful hybridization and analysis. METHODS In this research, we developed a three-dimentional stable and mechanically reliable microarray substrates by coating of two polymeric layers on standard microscope glass slides. For fabrication of these substrates, a thin film of ox...
متن کاملAn Efficient Covalent Coating on Glass Slides for Preparation of Optical Oligonucleotide Microarrays
Objective(s): Microarrays are potential analyzing tools for genomics and proteomics researches, which is in needed of suitable substrate for coating and also hybridization of biomolecules. Materials and Methods: In this research, a thin film of oxidized agarose was prepared on the glass slides which previously coated with poly-L-lysine (PLL). Some of the aldehyde groups of the activated aga...
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ژورنال
عنوان ژورنال: Microscopy Today
سال: 2001
ISSN: 1551-9295,2150-3583
DOI: 10.1017/s1551929500056480